Intron loss from the mitochondrial cox2 gene in 32 flowering plants does loss of the cox2i1 intron involve a cDNA/RNA intermediate? by Junjun Ouyang

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  • Plant genetics,
  • Introns,
  • Plant mitochondria,
  • Mitochondrial DNA

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Statementby Junjun Ouyang
The Physical Object
Paginationvii, 83 leaves :
Number of Pages83
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Open LibraryOL15255188M

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The mitochondrial NADH:ubiquinone oxidoreductase complex (Complex I) is a large protein complex formed from both nuclearly and mitochondrially encoded subunits. Subunit ND1 is encoded by a mitochondrial gene comprising five exons, and the mature transcript requires four RNA splicing events, two of which involve trans -splicing independently Cited by: Flowering plants have only a single mitochondrial group II intronic maturase gene (matR) and it is located in the final intron of nad1.

Its biochemical activity has not yet been demonstrated, however it gives rise to stable (and edited) RNA and shows evolutionary constraint on its amino acid sequence (Thomson, Macfarlane, Beagley Cited by: Convergent intron loss in the plastid genome has been reported at a broad phylogenetic level in the flowering plants, but very few cases are known among closely related taxa.

Phylogenetic Distribution of Mitochondrial Gene Losses in the Streptophyta. Events of gene loss inferred from the mitochondrial genome sequences of six green plants were mapped on the tree shown in Figure 3A. A repertoire of 72 genes was predicted for the mitochondrial genome of the green algal ancestor of Mesostigma and the five streptophytes Cited by: HGT followed by gene conversion between the foreign and native cox2 copies has been proposed as a mechanism of intron loss in Magnolia mitochondrial DNA (Hepburn et al., ).

Novel chimeric gene structures often arise from HGT and subsequent gene conversion between foreign and native sequences in angiosperm mitochondrial genomes (Barkman et Cited by: The Role of Horizontal Transfer in Shaping the Plant Mitochondrial Genome 55 The mitochondrial genome, on the other hand, is very receptive to foreign DNA, at least in angiosperms.

Often during flowering plant evolution, ribosomal protein genes have been lost from the mitochondrion and transferred to the nucleus. Here, we show that substitution by a duplicated, divergent gene originally encoding the chloroplast or cytosolic ribosomal protein counterpart accounts for two missing mitochondrial genes in diverse angiosperms.

The rps13 gene is missing from the mitochondrial. Forty-six species of diverse land plants were investigated by sequencing for their intron content in the mitochondrial gene nad1. A total of seven introns, all belonging to group II, were found. Gene content is highly variable, particulary in flowering plants where there has been rampant gene loss and gene transfer to the nucleus during angiosperm evolution.

Ribosomal protein genes have been lost much more often than most respiratory by: 3. Comparative genomics among gymnosperms suggested extensive loss of mitochondrial RNA editing sites from Welwitschia mirabilis based on predictive analysis.

However, empirical or transcriptome data to confirm this massive loss event are lacking, and the potential mechanisms of RNA site loss are unclear. By comparing genomic sequences with. There is no doubt that the intron has a patchy distribution among angiosperms.

For instance, in the most species-rich analysis of this intron, Sanchez-Puerta et al. found that 25% ( of ) of the sampled angiosperms contained the intron. At a finer scale, the most comprehensive examination within a single family found the intron in only 4% (17 of ) of Solanaceae Cited by: Adams KL, Daley DO, Qiu YL, Whelan J and Palmer JD () Repeated, recent and diverse transfers of a mitochondrial gene to the nucleus in flowering plants.

Nature – PubMed CrossRef Google ScholarCited by: The Vigna mitochondrial genome contains a conserved set of 17 cis-spliced and five trans-spliced group II introns (Fig.

1).Seed plant mitochondrial genomes typically require trans-splicing of the intron separating exons 3 and 4 of the nad5 gene to create a full-length nad5 transcript. In Vigna, exon 3 is identically oriented and less than 3 kb apart from exon 4 (Fig. 1), raising the Cited by:   The evolutionarily conserved YidC/Oxa1/Alb3 proteins are involved in the insertion of membrane proteins in all domains of life.

In plant mitochondria, individual knockouts of OXA1a, OXA2a, and OXA2b are embryo-lethal. In contrast to other members of the protein family, OXA2a and OXA2b contain a tetratricopeptide repeat (TPR) domain at the by: 4. Coexpression of an organellar gene in two different cellular genetic compartments has only been reported for a few mitochondrial genes (cox2, rpl5, sdh4) in land plants (Adams et al., ; Sandoval et al., ; Choi et al., ).

In our study, none of the 18 species tested showed coexpression of n-accD and by: Extensive gene loss and IGT of organelle DNA to the nucleus occurred in the early stages of endosymbiosis [].Nuclear genome sequences that originate from the mitogenome and plastome are referred to as nuclear mitochondrial DNA sequences (NUMTs) and nuclear plastid DNA sequences (NUPTs), respectively [12,13].Transfer of mitochondrial DNA to the nuclear Author: In-Su Choi, Tracey A.

Ruhlman, Robert K. Jansen, Robert K. Jansen. Intergenomic gene transfer (IGT) is continuous in the evolutionary history of plants. In this field, most studies concentrate on a few related species. Here, we look at IGT from a broader evolutionary perspective, using 24 plants.

We discover many IGT events by assessing the data from nuclear, mitochondrial and chloroplast genomes. Thus, we summarize the two roles of Cited by: RNA editing in plants alters specific nucleotides from C to U in mRNAs in plastids and in mitochondria. I here characterize the nuclear gene MITOCHONDRIAL EDITING FACTOR9 (MEF9) that is required for RNA editing of the site nad7 in the nad7 mitochondrial mRNA in Arabidopsis (Arabidopsis thaliana).

The MEF9 protein belongs to the E subfamily of. In plants, mitochondrial composition is relatively complex and flexible and has specific pathways to support photosynthetic processes in illuminated leaves.

Plant mitochondria also play important roles in a variety of cellular processes associated with. O X-T OX MODEL. The endosymbiotic theory, as explicated by Margulis (), was an eclectic formulation that concerned much about cellular evolution besides the origins of mitochondria and plastids, but convention has reduced the common use of thethe standard model to describe the origins of mitochondria was quite specific: the endosymbiont was identified as an Cited by: Mitochondrial biogenesis as revealed by mitochondrial transcript profiles during germination and early seedling growth in wheat mitochondrial cox2 gene was also not detected by our macroarray, although a blast (Oryza sativa L.) mitochondrial genome: frequent DNA sequence acquisition and loss during the evolution of flowering plants.

Mol Cited by: Plants were routinely grown in × cm plastic Petri dishes, on 1 Loss of Two Introns from the Magnolia tripetala Mitochondrial cox2 Gene Implicates Horizontal Gene Transfer and Gene Conversion as a Novel Mechanism of Intron Loss, Molecular Biology and Evolution, 29, 10, (), ().

Crossref. Stephan Greiner Cited by: Acknowledgements. We thank Alan Christensen, Volker Knoop, Sally Mackenzie, David Smith, Jonathan Wendel and Paul Wolf for providing comments and corrections to improve the manuscript and Wenhu Guo for help in compiling the intron distribution information in Fig.

Research in the Mower Lab on plant mitochondria is funded by the National Science. Indeed, the discovery of an intron between an addressing sequence and a mitochondrial gene that had been transferred to the nucleus in some plants confirms this expectation (45, ).

Accordingly, it is conceivable that one reason that splicing systems spread through primitive eukaryotic nuclear genomes was to satisfy the need to tag newly Cited by:   SUMMARY The endosymbiotic theory for the origin of mitochondria requires substantial modification.

The three identifiable ancestral sources to the proteome of mitochondria are proteins descended from the ancestral α-proteobacteria symbiont, proteins with no homology to bacterial orthologs, and diverse proteins with bacterial affinities not derived from α Cited by: Duplicated genes are considered as raw materials for evolutionary innovations.

They are common in eukaryotic genomes, particularly in plants due to the high incidence of whole genome duplication. Thus, understanding the factors that contribute to the retention of duplicated genes is a fundamental topic in evolutionary biology. I tackle this topic by examining how reciprocal Author: Shao-Lun Liu.

Sequence analysis of the first intron in the mitochondrial gene cox2 and its application in phylogenetic study. American Journal of Botany 84 Suppl. 6:. Full text of "Molecular biology and biotechnology of plant organelles: chloroplasts and mitochondria" See other formats.

Mitochondrial DNA suggests 12 origins of parasitism in angiosperms and implicates parasitic plants as vectors of horizontal gene transfer: Conference Wide: Barkman, Todd J., McNeal, Joel R., Lim, Seok-Hong, Coat, Gwen, Croom, Henrietta, Young, Ned, dePamphilis, Claude W.

mt DNA holoparasite horizontal gene transfer angiosperm phylogeny: 32 GENES IN CONFLICT,FLITTERS y Tyª T #OMPLEMENTINGgS T Tyª LEMENTINGgS T Figure The relative proportion of different-sized litters produced by 3 crosses. Dotted line: cross between (mostly) wildtype mice.

Black bars: cross between +/t indi-viduals in which the t is Size: 6MB. RNA-editing in chloroplasts and mitochondria of plants. Conclusion.

References. Part 2. New Hypothetical Mechanisms and Contours of The New Paradigm. P 24 Virus-Induced Gene Silencing is a highly effective tool for gene function studies in phylogenetically valuable species Wege S., Lange S., Stammler A. and Becker A. University of Bremen, Germany; [email protected] Extremely long survival of a nad7 pseudogene and peculiar rearrangements in the mitochondrial DNA of liverworts Wahrmund U.

Detailed analysis of the mitochondrial rpl27 gene in the O. sativa nuclear genome shows that the rpl27 gene acquired a promoter sequence and 5-UTR via inter- and intrachromosomal duplications from the O.

sativa sptrelated (Osspt16) gene, which is a homologue of the S. cerevisiae spt16 gene (Fig. The RII gene is considered nonessential, in that it is not needed to growth in most E.

coli host. However, RII is essential for a T4 phage that infects an E. coli host harboring a lambda prophage (or its lambda defective). The RII gene represents a class of ‘accessory’ genes which are well conserved in clinical isolates of T4 from   alleles to intron-lacking alleles of the same gene in genetic crosses.

This intron has been detected in the mitochondrial cox1 gene of 48 angiosperms out of tested. Based on sequence data for the intron and the host genome, it appears that this intron has been independently acquired by cross-species horizontal. Science – Hu G, Gong A, Wang Y, Ma S, Chen X, Chen J, Su C, Shibata A, Strauss-Soukup J, Drescher K et al () LincRNA-Cox2 promotes late inflammatory gene transcription in macrophages through modulating SWI/SNF-mediated chromatin remodeling.

J Immunol –   Horizontal acquisition of multiple mitochondrial genes from a parasitic plant followed by gene conversion with host mitochondrial genes.

PubMed Central. Background Horizontal gene transfer (HGT) is relatively common in plant mitochondrial genomes but the mechanisms, extent and consequences of transfer remain largely unknown. Previous. Determining mycotoxins and mycotoxigenic fungi in food and feed known include the ergot alkaloids from Claviceps purpurea and C.

paspali, but since the outbreaks of ‘St. Anthony’s fire’ in the middle ages many other mycotoxins were discovered, for example T-2 toxin during World War II and inwhen mycotoxins became famous by the.

Full text of "Progress in Botany [electronic resource]: Genetics Cell Biology and Physiology Systematics and Comparative Morphology Ecology and Vegetation Science" See other formats.

The insert was mapped and partially sequenced to determine the intron-exon boundaries. The exons contained in the phage clone correspond to exons 5 to 8 of the human gene (15). To construct the targeting vector a 5′-homology arm of kb containing exon 6 a 3′-homology arm of kb containing the 3′ end of exon 8 the cassette and the.

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PLOS ONE 5: e Krieger U, Lippman ZB, Zamir D () The flowering gene single flow-er truss drives heterosis for yield in tomato. Nat Genet – resources for File Size: 16MB.The study was initiated by cloning CbCyp51, which is a single copy 1,bp intron-free gene with homology to other fungal Cyp51 genes.

Because resistance to DMIs can be related to polymorphism in promoter or coding sequences, >2, nucleotides were sequenced encompassing CbCyp51 coding and flanking regions from isolates with varying EC

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